96 well plates microamptm optical 96-well reaction plate with barcode Search Results


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Thermo Fisher 96 well plate
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Bio-Rad hard shell pcr hsp9645 plate
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Bio-Rad multiplate pcr platetm
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Promega nuclease-free water
Troubleshooting Guide for Using Murine PCLS
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EuroClone framestar 96-well pcr plates
Troubleshooting Guide for Using Murine PCLS
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Bio-Rad ssofasttm evagreen supermix
Troubleshooting Guide for Using Murine PCLS
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Qiagen rneasy micro kit
Troubleshooting Guide for Using Murine PCLS
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Troubleshooting Guide for Using Murine PCLS

Journal: Current Protocols

Article Title: The Use of Precision‐Cut Lung Slices for Studying Innate Immunity to Viral Infections

doi: 10.1002/cpz1.505

Figure Lengend Snippet: Troubleshooting Guide for Using Murine PCLS

Article Snippet: PCLS (see Basic Protocol ) TRIzol (Invitrogen, cat. no. 15596018) Chloroform RNeasy Mini kit (Qiagen, cat. no. 74106) Nuclease‐free water (Promega, cat. no. P1193) High‐Capacity RNA‐to‐cDNA kit (Thermo Fisher, cat. no. 4387406) Quantitect Probe PCR Master Mix (Qiagen, cat. no. 204345) Target primers (see Table ) 1.5‐ml Eppendorf tubes Tissue homogenizer (TissueLyser LT, Qiagen) Gel Eppendorf tubes (VWR, cat. no. 733‐2478) Refrigerated microcentrifuge, 4°C Spectrophotometer (NanoDrop 1000 Spectrophotometer, Thermo Scientific) 96‐well PCR plate (MicroAmpTM Optical 96‐Well Reaction Plate, Applied Biosystems, cat no. N8010560) 7500 Fast Real‐Time PCR System (Applied Biosystems) 7500 Fast System SDS Software (Applied Biosystems)

Techniques: Concentration Assay